Annex A - Group Research Proposal

Group Project Proposal (Science)


Names: Xuan Yi, Caleb, Li Kai
Class: S2-02
Group Reference: F

A.    Indicate the type of research that you are adopting:

[ X ] Test a hypothesis: Hypothesis-driven research
[    ] Measure a value: Experimental research (I)JA
  ] Measure a function or relationship: Experimental research (II)
[    ] Construct a model: Theoretical sciences and applied mathematics
[    ] Observational and exploratory research
  1. Type & Category

Type of research: 1 (Test a hypothesis) (Write down one number from 1 to 6)

Category  –   18 (Microbiology) (Write down one number from 7 to 20)

Sub-category – C (Bacterial Microbiology) (Write down the sub-heading alphabet)

Application of project relevant to SST Community, Society or the World:
We would be able to test if mouthwash is really useful in helping us reduce the amount of bacteria in our mouth.
C.    Write down your research title:
Investigation of how the use of mouthwash affects the number of visible major bacteria colonies.

D.   (a) Aim / question being addressed 

How does the presence of mouthwash affect the amount of bacteria in our mouth?

(b) Independent variable
The independent variable will be the presence of mouthwash.

(c) Dependent variable
The dependant variable is the amount of bacteria in the agar plates.

(d) Controlled variables
  • Volume of mouthwash
  • Duration of which agar plates were incubated
  • Environment - The type of agar media and the temperature in the incubator is mostly kept constant and provides good growing environment for the bacteria.
      (e) Hypothesis

The swab we did without mouthwash would have more bacteria than the one swabbed with mouthwash.

E.    Method – Description in detail of method or procedures (The following are important and key items that should be included when formulating ANY AND ALL research plans.)

  • (a) Equipment list:
  • 6 agar plates
  • Cotton swabs
  • Saliva samples
  • Mouthwash
  • Spirit lamp
  • Pipette
  • Test Tube
(b) Procedures: Detail all procedures and experimental design to be used for data collection
  1. Light up the spirit lamp
  2. Place the equipment near the flame to ensure that it does not get contaminated.
  3. Take a cotton swab and swipe the inside of your cheek, trying to get as much of the surface of the swab covered with saliva.
  4. Pipette out 10 millilitres of sterile water into the test tube
  5. Dip the swab in a test tube into the test tube.
  6. Use the pipette and extract 100 microlitres of water and put in on the agar plate.
  7. Use the spreader and spread the sample all over the agar plate.
  8. Rinse your mouth with mouthwash for 30 seconds.   
  9. Repeat steps 3 to 6 for the mouthwash.
  10. Cover the agar plates and wrap around the plate with Parafilm.
  11. With the plates agar facing upwards, place the agar plates in the incubator.
(c) Risk, Assessment and Management: Identify any potential risks and safety precautions to be taken.

Cotton swabs may not be sterile, unwanted bacteria may enter our mouth.
Spit out the saliva after swiping the swab your mouth into an appropriate place.
We might cut and injure ourselves when transporting the agar plates and drops them.
Be careful when transporting the agar plates and just let 1 person take them.
We might burn ourselves from  the spirit lamp used to prevent contamination from entering our setup.
Be careful when near/dealing the fire. Always follow the instructions from the lab tech/ teacher and extinguish the fire when not in use.
Infection from the bacteria
Take safety precautions and do not come into contact with the bacteria colonies.

(d) Data Analysis: Describe the procedures you will use to analyze the data/results that answer research questions or hypotheses
  1. Take the agar plate out of the incubator.
  2. Count the number of colonies in the agar plate if there are any.
  3. Repeat the counting process once a day, over a period of 5 days.
  4. After doing so, compare the number of colonies between each day and come to a conclusion.

F. Bibliography: List at least five (5) major sources (e.g. science journal articles, books, internet sites) from your literature review. If you plan to use vertebrate animals, one of these references must be an animal care reference. Choose the APA format and use it consistently             to reference the literature used in the research plan. List your entries in alphabetical order for each type of source.
a) Books

b) Journals

Corner, T. R.. (1984). Do Mouthwashes Really Kill Bacteria?. The Science Teacher, 51(6), 34–40. Retrieved from

(c) Websites

Bryner, J. (2012). Do Mouthwashes Work? Retrieved February 15, 2016, from

Summer Science Academy Experiment:. (n.d.). Retrieved February 15, 2016, from

Mouthwash Science Fair Experiment. (n.d.). Retrieved February 15, 2016, from

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